20.Mittraphab Yanisa（生物資源環境科学府・博士課程）

Protective effects of acetone extract from Entada phaseoloides leaves on UVB-irradiated HaCaT keratinocytes

Entada phasioloides was reported to have triterprene, saponin, and phenolic compounds, which has protective effect on UVB-irradiation and anti-inflammation. In southeast Asia, stems and seeds of E. phaseoloides have been used as soap, as well as traditional medicine to treat skin diseases. However, the leaves of E. phaseoloides are barely reported their biological activites. In this study, we investigated the protective effect of acetone extract from E. phaseoloides leaves (AE) on UVB-irradiated human epidermal keratinocytes (HaCaT cells). The results show that AE, which is effective at 40 µg/mL, has nontoxic effect on normal keratinocyte HaCaT cells. UVB damage (30 mJ/cm2) resulted significant reduction in cell viability in a time dependent manner. HaCaT cells were pretreated with AE for 24 h and the irradiated with UVB rays. The results showed that the AE prevented UVB-induced cytotoxicity which was verified by MTT assay.